Detection of Clostridium difficile toxin : 4 5 Comparison of enzyme immunoassays 6 7 with results obtained by cytotoxicity assay 8 9 10 11 12
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چکیده
10 11 12 Daniel M. Musher,* Atisha Manhas, Pranav Jain, Franziska Nuila, 13 14 Amna Waqar, Nancy Logan, Bernard Marino, Edward A. Graviss, 15 16 17 From the Medical Service (Infectious Disease Section), Michael E. DeBakey 18 Veterans Affairs Medical Center, Houston, Texas, the Departments of Medicine 19 and Molecular Virology and Microbiology, Baylor College of Medicine, and the 20 Department of Pathology, Baylor College of Medicine, Houston, Texas 21 22 These data were presented in preliminary form at the 44th annual meeting of the 23 Infectious Disease Society of America, October, 2006 24 25 Running title: Detection of C. difficile toxin 26 27
منابع مشابه
Detection of Clostridium difficile toxin: comparison of enzyme immunoassay results with results obtained by cytotoxicity assay.
Several kinds of laboratory techniques are available to detect Clostridium difficile toxin in fecal samples. Because questions have been raised about the reliability of immunoassays compared to the accepted standard, cytotoxicity assay, we studied three enzyme immunoassays (EIAs) and one rapid EIA, which demonstrated relatively good sensitivities and specificities compared to cytotoxicity assay.
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AIM To evaluate a new enzyme immunoassay (EIA) method for detection of Clostridium difficile toxin by comparing it to cytotoxicity assay. To investigate the nature of false negative and false positive EIA results by evaluating clinical and therapeutic parameters. METHODS 737 consecutive diarrhoeal specimens collected from patients clinically suspected of having C difficile colitis were tested...
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Clostridium difficilewas first isolated in 1935 in the normal flora of newborns.1 Since 1978, it has been reported to be a cause of antimicrobial-associated diarrhea, colitis, and pseudomembranous colitis.2 During the late 1970s and early 1980s, several studies concluded that two toxins, toxin A (enterotoxin) and toxin B (cytotoxin), were responsible for the characteristic symptoms of C. diffic...
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Background & Aims: Clostridium difficile has been identified as a pathogen in antibiotic associated diarrhea (AAD), pseudomembranous colitis and also nosocomial diarrhea. The present study was performed to find the prevalence of toxigenic strains of C .difficile isolated from diarrhea patients hospitalized in Tehran hospitals. Method: A total of 98 fecal samples obtained during July to December...
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